Sperm Function

1、Sperm DNA fragmentation test

Meaning of the test

● Assess male fertility potential. Analyze the etiology of infertility.

● Evaluate ART outcome (fertilization rate, blastocyst formation).

● Predict male-factor miscarriage and assist evidence-based treatment.

● Measure adverse effect of elevated ROS on sperm.

Technology Highlights

Damaged sperm chromatin formed single-stranded DNA after acidification, and the single-stranded DNA combined with the dye acridine orange showed orange or red fluorescence by 488 nm laser excitation; whereas normal sperm chromatin retained the intact double-stranded structure of DNA, and combined with the dye acridine orange showed green fluorescence by 488 nm laser excitation. An increase in the percentage of red light after acidification of semen samples indicates an increase in chromatin structure abnormalities.

Features:

1.International standardized flow-cytometric technique, providing accurate results.

2.Testing rapidly, with 2 min/test.

3.Present multiple parameters reports(DFI, HDS).

4.A large amount of sperm detected, the results have good reproducibility.

5.Required device: flow cytometer (blue excitation).

2、Sperm mitochondrial staining kit

Meaning of the test

● Assessing sperm motility and fertilization potential: Sperm mitochondrial function directly impacts energy supply for sperm movement; detecting its activity (e.g., membrane potential) evaluates sperm vitality and fertilization capability.

● Diagnosing causes of male infertility: Mitochondrial dysfunction is a key factor in asthenozoospermia or azoospermia; testing helps identify metabolic origins of infertility.

● Optimizing assisted reproductive techniques (ART): Selecting sperm with intact mitochondrial function for ICSI may enhance embryonic development success rates.

Technology Highlights

JC-1 is a carbocyanine-based fluorescent dye used to detect the mitochondrial membrane potential ΔΨm, and exists in two different forms, polymers (J-aggregates) and monomers (monomers), in the cell. When the mitochondrial membrane potential is high, JC-1 accumulates in the mitochondrial matrix and forms polymers, which can produce orange-red fluorescence. When the mitochondrial membrane potential is low, JC-1 can not be aggregated in the mitochondrial matrix, and then JC-1 is a monomer, which can produce green fluorescence.

Features:

Adopt international JC-1 probe.

Report the percentage of sperm with high mitochondrial membrane potential with significant clinical application.

Required equipment: flow cytometer (blue excitation).

3、Sperm acrosome reaction test kit

Meaning of the test

1. Globally leading commercialized kit for inducing sperm acrosome reaction.

2. It is mainly used to analyze the etiology of infertility , investigate the causes of low IVF fertilization rate,and predict fertilization outcome.

3.Critical indicator for selecting IUI/IVF/ICSI

4.The standardized test method in accordance with WHO technical specifications is adopted, and theaccuracy and repeatability of the results are more guaranteed.

5. Adopting advanced targets that comply with the WHO Manual for Human Sperm Testing Laboratories——Anti-CD46- FITC

Technology Highlights

The acrosome reaction is a key process in the fertilisation of sperm and egg, including the fusion of plasma membrane and acrosomal membrane, secretion of enzymes, etc. If the acrosome reaction is insufficient or abnormal, it will lead to male infertility. Sperm without acrosome reaction may have a complete acrosome, or it may have a missing acrosome that does not fluoresce. And sperm with acrosome reaction exposed CD46 antigen on the acrosome membrane, which specifically binds to FITC conjugated CD46 antibody and emits green fluorescence.